Journal Article

The Promoter for the Maize C<sub>4</sub> Pyruvate,orthophosphate Dikinase Gene Directs Cell- and Tissue-Specific Transcription in Transgenic Maize Plants

Mitsutaka Taniguchi, Katsura Izawa, Maurice S.B. Ku, Jenq-Hong Lin, Hideaki Saito, Yuji Ishida, Shozo Ohta, Toshihiko Komari, Makoto Matsuoka and Tatsuo Sugiyama

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 1, pages 42-48
Published in print January 2000 | ISSN: 0032-0781
Published online January 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/41.1.42
The Promoter for the Maize C4 Pyruvate,orthophosphate Dikinase Gene Directs Cell- and Tissue-Specific Transcription in Transgenic Maize Plants

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  • Molecular and Cell Biology
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The pyruvate,orthophosphate dikinase (PPDK) gene coding the chloroplast enzyme involved in C4 photosynthesis has a dual promoter system. The first promoter is responsible for the transcription of a larger transcript and its product is targeted to the chloroplast (hence, it is designated as C4Pdk promoter) while the second promoter is responsible for the transcription of a smaller transcript and its product remains in the cytosol. In this study, chimeric maize C4Pdk promoter (0.9 or 1.5 kb)-β-glucuronidase or luciferase fusion genes were introduced into maize plants by Agrobacterium-mediated transformation. The cell- and tissue-specificities of the maize C4Pdk promoter in the transgenic maize plants were examined by histochemical and enzymic activity analyses of the reporters in different photosynthetic cells and tissues. The results showed that the reporter proteins are almost exclusively localized in leaf mesophyll cells. Among the tissues tested, leaf blade had the highest reporter activities with sheath exhibiting about 10% of the activities in blade. Husk, stem, tassel and root had no or very little reporter activities. Taken together, these results suggest that the maize C4Pdk promoter is specifically transcribed in the mesophyll cells of leaf blade and to a much less extent in the mesophyll cells of sheath, but not in leaf bundle sheath cells or other tissues. Furthermore, the 0.9 kb maize C4Pdk promoter sequences appear to contain the necessary cis-acting elements for its cell- and organ-specific expression.

Keywords: C4 photosynthesis; Maize (Zea mays); Promoter; Pyruvate,orthophosphate dikinase; Transgenic plant

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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