Journal Article

Isolation and Characterization of Arsenate-Sensitive and Resistant Mutants of <i>Chlamydomonas reinhardtii</i>

Shoko Fujiwara, Isao Kobayashi, Satoshi Hoshino, Toshikazu Kaise, Kosuke Shimogawara, Hideaki Usuda and Mikio Tsuzuki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 1, pages 77-83
Published in print January 2000 | ISSN: 0032-0781
Published online January 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/41.1.77
Isolation and Characterization of Arsenate-Sensitive and Resistant Mutants of Chlamydomonas reinhardtii

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Arsenate-sensitive and resistant mutants of Chlamydomonas reinhardtii were obtained by screening mutants generated by random insertional mutagenesis for growth in the presence of various concentrations of arsenate. The intracellular concentrations of arsenic in the mutants kept in the arsenate-containing medium were determined with an atomic absorption spectrophotometer. The intracellular levels of arsenic in the arsenate-resistant mutants were all lower than that of the parent strain CC425. Some of the arsenate-sensitive mutants, AS1 and AS3, showed obviously higher levels of arsenic than that of CC425, while other sensitive mutant, AS2, did not accumulate arsenic so much. Analysis of the chemical species of arsenic suggested that inorganic arsenic was converted to dimethylarsinic acid (DMAA) in CC425. However, DMAA was hardly detected in AS2. The mechanisms of the resistance to arsenate are discussed on its uptake and detoxification.

Keywords: Arsenate-resistant; Arsenate-sensitive; Biomethylation; Chlamydomonas reinhardtii; Dimethylarsinic acid

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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