Journal Article

An RNA-Binding Protein, AtRBP1, Is Expressed in Actively Proliferative Regions in <i>Arabidopsis thaliana</i>

Masashi Suzuki, Atsushi Kato and Yoshibumi Komeda

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 3, pages 282-288
Published in print March 2000 | ISSN: 0032-0781
Published online March 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/41.3.282
An RNA-Binding Protein, AtRBP1, Is Expressed in Actively Proliferative Regions in Arabidopsis thaliana

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A cDNA clone, named XF41, that encodes an RNA-binding protein was isolated from Arabidopsis thaliana. The deduced protein, named AtRBP1, contains two conserved consensus sequence-type RNA-binding domains (CS-RBDs) in the N-terminal half, a putative PY motif (a target of a WW domain) in the center, and uncharacterized C-terminal domain. A binding assay demonstrated that the AtRBP1 can bind to single-stranded nucleic acids in vitro. Analysis of localization of the GUS activity of transgenic Arabidopsis thaliana plants that have the chimeric gene containing the upstream sequence of the AtRBP1 gene and GUS gene demonstrated that the AtRBP1 gene is expressed in meristematic tissues such as the vegetative shoot apex and root tips, developing organs such as floral buds and pistils of young flowers, abscission layers of immature siliques and junctions of pedicels. Considering the specificity of the expression, AtRBP1 may be required in the progress of cell proliferation.

Keywords: Arabidopsis thaliana; Cell proliferation; PY motif; RNA-binding protein

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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