Journal Article

Inhibition of Cell-Plate Formation by Brefeldin A Inhibited the Depolymerization of Microtubules in the Central Region of the Phragmoplast

Hiroki Yasuhara and Hiroh Shibaoka

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 3, pages 300-310
Published in print March 2000 | ISSN: 0032-0781
Published online March 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/41.3.300
Inhibition of Cell-Plate Formation by Brefeldin A Inhibited the Depolymerization of Microtubules in the Central Region of the Phragmoplast

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Treatment of tobacco BY-2 cells with 20 üM brefeldin A (BFA), which causes disassembly of the Golgi apparatus (Yasuhara et al. 1995), completely inhibited the formation of the cell plate when the treatment was started before the chromosomes had begun to to condense. In cells in which cell-plate formation was inhibited by BFA, the centrifugal development of the phragmoplast was also inhibited. In such cells, the depolymerization of microtubules in the central region of the phragmoplast did not occur at least for 1 h after the formation of the phragmoplast, while the centrifugal development of the phragmoplast and cell-plate formation were completed in almost all cells not treated with BFA. The inhibition of cell-plate formation seems to inhibit the centrifugal development of the phragmoplast by inhibiting the depolymerization of microtubules in the central region of the phragmoplast, which is required for the supply of free tubulin necessary for the polymerization of microtubules at the outer margins of the phragmoplast.

Keywords: Brefeldin A; Cell plate; Cytokinesis; Phragmoplast; Tobacco BY-2 cells

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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