Journal Article

Chloroplast Tubules Visualized in Transplastomic Plants Expressing Green Fluorescent Protein

Takashi Shiina, Keiko Hayashi, Nao Ishii, Kazuya Morikawa and Yoshinori Toyoshima

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 3, pages 367-371
Published in print March 2000 | ISSN: 0032-0781
Published online March 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/41.3.367
Chloroplast Tubules Visualized in Transplastomic Plants Expressing Green Fluorescent Protein

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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A fusion between the plastid psbA promoter and the green fluorescent protein gene (gfp) was introduced into the tobacco chloroplast genome by stable plastid transformation. GFP was synthesized actively and exclusively in the chloroplasts. Tubular projections filled with GFP but containing no chlorophyll were visualized for the first time in chloroplasts of these transplastomic plants. Occasionally, the tubules connect chloroplasts with each other, suggesting the possibility of the exchange of endogenous proteins. However, the fusion of protoplasts between the transplastomic and wild-type plants showed that such chloroplast connections might be rare in mesophyll protoplasts.

Keywords: Chloroplast; Chloroplast transformation; Chloroplast tubule; Green fluorescent protein; Nicotiana tobaccum; psbA promoter

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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