Journal Article

Wound-Induced Ethylene Synthesis and Expression and Formation of 1-Aminocyclopropane-1-Carboxylate (ACC) Synthase, ACC Oxidase, Phenylalanine Ammonia-Lyase, and Peroxidase in Wounded Mesocarp Tissue of <i>Cucurbita maxima</i>

Masaya Kato, Yoshitaka Hayakawa, Hiroshi Hyodo, Yoshinori Ikoma and Masamichi Yano

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 4, pages 440-447
Published in print April 2000 | ISSN: 0032-0781
Published online April 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/41.4.440
Wound-Induced Ethylene Synthesis and Expression and Formation of 1-Aminocyclopropane-1-Carboxylate (ACC) Synthase, ACC Oxidase, Phenylalanine Ammonia-Lyase, and Peroxidase in Wounded Mesocarp Tissue of Cucurbita maxima

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1-Aminocyclopropane-1-carboxylate (ACC) synthase was rapidly induced in mesocarp tissue of Cucurbita maxima after wounding in the cut surface layer in 1 mm thickness (ca. 9 cells) (first layer) in both the enzyme activity and the levels of transcript. This led to a rapid accumulation of ACC and hence ethylene production. In the inside tissue (1–2 mm) (second layer), no significant induction of ACC synthase was observed, which resulted in a low level of ACC, although ethylene was evolved at a much lower rate than the first one. In contrast to ACC synthase, ACC oxidase was induced markedly in both the first and second layers and the development of its activity and the levels of mRNA remained high until later stages. It was considered that wound ethylene was closely associated with the development of ACC oxidase, since 2,5-norbornadiene (NBD), an inhibitor of ethylene action, substantially suppressed it. Phenylalanine ammonia-lyase (PAL) greatly increased in activity after wounding similarly to that of ACC synthase, in which increase in PAL activity occurred predominantly in the first layer. Induction of peroxidase activity after wounding had a close correlation in profile with that of ACC oxidase in that marked increases in the activity were observed in both the first and second layers and were strongly suppressed by NBD application. Four peroxidase isozymes were found by PAGE, among which a fraction was newly detected after wounding.

Keywords: ACC oxidase; ACC synthase; Cucurbita maxima; Peroxidase; Phenylalanine ammonia-lyase; Wound-induced ethylene

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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