Journal Article

Isolation of the Gene for EILP, an Elicitor-Inducible LRR Receptor-Like Protein, from Tobacco by Differential Display

Daigo Takemoto, Makoto Hayashi, Noriyuki Doke, Mikio Nishimura and Kazuhito Kawakita

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 4, pages 458-464
Published in print April 2000 | ISSN: 0032-0781
Published online April 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/41.4.458
Isolation of the Gene for EILP, an Elicitor-Inducible LRR Receptor-Like Protein, from Tobacco by Differential Display

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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We screened tobacco genes, which are differentially expressed in response to a fungal elicitor, and have isolated a gene which codes for a leucine-rich repeat (LRR) protein closely related to Cf genes in tomato. The EILP (elicitor inducible LRR protein) gene encodes 95 kDa protein, which consists of a putative membrane spanning region, 28 leucine-rich repeats and some N-linked glycosylation sites, and shows high homology to Cf-2/Cf-5 family genes. Southern blot analysis revealed the presence of some genes homologous to EILP in tobacco, like Cf genes in tomato. The expression of EILP was low at the basal level and increased by treatment with elicitor, implying that EILP is involved in both preexisting and inducible surveillance systems. The expression of EILP was activated by a nonpathogen, Pseudomonas syringae pv. glycinea, and in a delayed fashion by the tobacco pathogen P. syringae pv. tabaci, suggesting that the product of EILP may be involved in non-host disease resistance in tobacco.

Keywords: Differential display; Elicitor-inducible gene; LRR protein; Pseudomonas syringae; Tobacco

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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