Journal Article

Three <i>Arabidopsis</i> Genes Encoding Proteins with Differential Activities for Cysteine Synthase and β-Cyanoalanine Synthase

Yube Yamaguchi, Tatsuo Nakamura, Tomonobu Kusano and Hiroshi Sano

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 4, pages 465-476
Published in print April 2000 | ISSN: 0032-0781
Published online April 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/41.4.465
Three Arabidopsis Genes Encoding Proteins with Differential Activities for Cysteine Synthase and β-Cyanoalanine Synthase

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Three cDNA clones encoding putative cysteine synthases (O-acetylserine (thiol) lyase, EC 4.2.99.8) were isolated from Arabidopsis thaliana and designated AtcysC1, AtcysD1 and AtcysD2, respectively. Southern blot analyses suggested that the corresponding genes were present as a single copy, or at most two copies, in the A. thaliana genome. Escherichia coli complementation analyses confirmed that the cDNAs encode cysteine synthase and the corresponding proteins produced in E. coli clearly showed cysteine synthase activity. In addition, AtcysC1 protein showed β-cyanoalanine synthase (EC 4.4.1.9) activity, but the other two did not. Kinetic analysis suggests that AtcysC1 actually functions as β-cyanoalanine synthase rather than cysteine synthase in vivo. The mRNA accumulation of AtcysC1, AtcysD1 and AtcysD2 differed in various organs, but did not change markedly when A. thaliana seedlings were subjected to various stresses, including nutrient deprivation. In vivo targeting experiments indicated that AtcysD1 and AtcysD2 are cytoplasmic isozymes, and AtcysC1 is a mitochondrial isozyme.

Keywords: Arabidopsis thaliana; β-cyanoalanine synthase; Cysteine synthase; Pyridoxal 5′-phosphate

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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