Journal Article

Molecular and Biochemical Characterization of a Novel Hydroxycinnamoyl-CoA: Anthocyanin 3-<i>O</i>-Glucoside-6″-<i>O</i>-Acyltransferase from <i>Perilla frutescens</i>

Keiko Yonekura-Sakakibara, Yoshikazu Tanaka, Masako Fukuchi-Mizutani, Hiroyuki Fujiwara, Yuko Fukui, Toshihiko Ashikari, Yasuyuki Murakami, Masaatsu Yamaguchi and Takaaki Kusumi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 4, pages 495-502
Published in print April 2000 | ISSN: 0032-0781
Published online April 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/41.4.495
Molecular and Biochemical Characterization of a Novel Hydroxycinnamoyl-CoA: Anthocyanin 3-O-Glucoside-6″-O-Acyltransferase from Perilla frutescens

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

We have isolated and characterized a cDNA, PfAT208, encoding hydroxycinnamoyl-CoA: anthocyanin 3-O-glucoside-6″-O-acyltransferase (3AT) from Perilla frutescens. The identity of the cDNA was established by determination of the reaction products with recombinant enzyme overexpressed in Escherichia coli. The deduced amino acid sequence has a few regions that are conserved in a CoA-dependent acyltransferase family. The recombinant enzyme produced in yeast could utilize cyanidin 3-glucoside and cyanidin 3,5-diglucoside, putative substrates in vivo, as well as other anthocyanins. The inhibitory effects of diethyl pyrocarbonate and N-ethylmaleimide on the recombinant 3AT activities suggest that histidine and cysteine residues are important for their catalytic function. These properties are in common with anthocyanin 5-O-glucoside-6″-O-acyltransferase (5AT). In Northern analysis, a transcript of PfAT208 was detected in the young leaves of perilla red forma. The properties of other cDNAs, gentian GAT106 and petunia PhAT48, isolated during the above cloning procedure are also described.

Keywords: Acyltransferase; Anthocyanin; Flavonoid; Gentiana triflora; Perilla frutescens; Petunia hybrida

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.