Journal Article

Evidence of Chlorophyll Synthesis Pathway Alteration in Desiccated Barley Leaves

Pascaline Le Lay, Philippe Eullaffroy, Philippe Juneau and Radovan Popovic

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 5, pages 565-570
Published in print May 2000 | ISSN: 0032-0781
Published online May 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/41.5.565
Evidence of Chlorophyll Synthesis Pathway Alteration in Desiccated Barley Leaves

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In etiolated leaves, saturating flash of 200 ms induces phototransformation of protochlorophyllide (Pchlide) F655 into chlorophyllide (Chlide), then into Chi through reactions which do not need light sensibilisation. The synthesis of Chi is known to be slowed down in etiolated leaves exposed to desiccation stress. In order to analyse the intensity and time-course of Chlide transformation into Chi, we used the fluorescence emission of etiolated leaves previously exposed to a 200 ms saturating flash. We used lowtemperature fluorescence spectroscopy to reveal the inhibition site of Chi synthesis in etiolated barley leaves exposed to water stress. Shibata shift appears as the main target point of the water deficit. It was found that water deficit inhibits partially active Pchlide F655 regeneration. Also, esterification of Chlide into Chi is impaired. It appears that these inhibitory effects alter the appearance of PSII active reaction centres.

Keywords: Barley; Chlorophyll biosynthesis; Chlorophyllide; Photosystem II assembly; Protochlorophyllide; Water-deficit

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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