Journal Article

Over-Expression of Tobacco <i>knotted 1</i>-Type Class1 Homeobox Genes Alters Various Leaf Morphology

Asuka Nishimura, Masanori Tamaoki, Tomoaki Sakamoto and Makoto Matsuoka

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 5, pages 583-590
Published in print May 2000 | ISSN: 0032-0781
Published online May 2000 | e-ISSN: 1471-9053 | DOI:
Over-Expression of Tobacco knotted 1-Type Class1 Homeobox Genes Alters Various Leaf Morphology

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry


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We compared the phenotypes of transgenic tobacco plants over-expressing various knotted1-type class1 homeobox genes. All transformants showed abnormal leaf morphology, with the degree of abnormality depending upon the Nicotiana tabacum homeobox (NTH) gene that was over-expressed. Tobacco plants over-expressing NTH1 or NTH9 showed a relatively weak phenotype, while NTH15 and NTH20 over-expressing plants exhibited severe alterations, with occasional ectopic shoot formation on the leaves. Plants over-expressing NTH22 had a relatively severe phenotype, but did not form any ectopic shoots. These results indicate that all of the NTH genes can influence leaf development from the shoot apical meristem, but that the effect varies with the gene. Based on phylogenetic analysis of the NTH genes and comparison of the phenotypes of plants over-expressing them, we suggest that the kn1-type class1 family can be divided into two subgroups, and that the differences in their ability to induce the abnormal phenotype corresponds to the structures of their conserved domains.

Keywords: knl-type homeobox family; KNOX domain; Leaf morphology; Transgenic plant; Tobacco

Journal Article.  0 words. 

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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