Journal Article

Transgenic Rice Plants Expressing a <i>Bacillus subtilis</i> Protoporphyrinogen Oxidase Gene Are Resistant to Diphenyl Ether Herbicide Oxyfluorfen

Hee Jae Lee, Sung Beom Lee, Jung Sung Chung, Sung Uk Han, Oksoo Han, Ja Ock Guh, Jong Seong Jeon, Gynheung An and Kyoungwhan Back

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 6, pages 743-749
Published in print June 2000 | ISSN: 0032-0781
Published online June 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/41.6.743
Transgenic Rice Plants Expressing a Bacillus subtilis Protoporphyrinogen Oxidase Gene Are Resistant to Diphenyl Ether Herbicide Oxyfluorfen

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Protoporphyrinogen oxidase (Protox), the penultimate step enzyme of the branch point for the biosynthetic pathway of Chi and hemes, is the target site of action of diphenyl ether (DPE) herbicides. However, Bacillus subtilis Protox is known to be resistant to the herbicides. In order to develop the herbicide-resistant plants, the transgenic rice plants were generated via expression of B. subtilis Protox gene under ubiquitin promoter targeted to the cytoplasm or to the plastid using Agrobacterium-mediated gene transformation. The integration and expression of the transgene were investigated at T0 generation by DNA and RNA blots. Most transgenic rice plants revealed one copy transgene insertion into the rice genome, but some with 3 copies. The expression levels of B. subtilis Protox mRNA appeared to correlate with the copy number. Furthermore, the plastidal transgenic lines exhibited much higher expression of the Protox mRNA than the cytoplasmic transgenic lines. The transgenic plants expressing the B. subtilis Protox gene at To generation were found to be resistant to oxyfluorfen when judged by cellular damage with respect to cellular leakage, Chi loss, and lipid peroxidation. The transgenic rice plants targeted to the plastid exhibited higher resistance to the herbicide than the transgenic plants targeted to the cytoplasm. In addition, possible resistance mechanisms in the transgenic plants to DPE herbicides are discussed.

Keywords: Key words: Diphenyl ether herbicides; Herbicide resistance; Oxyfluorfen; Protoporphyrinogen oxidase; Transgenic rice plants

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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