Journal Article

Molecular Cloning and Characterization of a cDNA Encoding Proline Transporter in Rice

Yumiko Igarashi, Yoshu Yoshiba, Tomoko Takeshita, Sayuri Nomura, Jun Otomo, Kazuko Yamaguchi-Shinozaki and Kazuo Shinozaki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 6, pages 750-756
Published in print June 2000 | ISSN: 0032-0781
Published online June 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/41.6.750
Molecular Cloning and Characterization of a cDNA Encoding Proline Transporter in Rice

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A cDNA encoding a proline (Pro) transporter (ProT) was isolated and characterized from a cDNA library prepared from 14-d-old seedlings of Oryza sativa cv. Akibare. The deduced amino acid sequence of the rice ProT protein (OsProT) had 68.8% homology to the ProT protein 1 from Arabidopsis thaliana and 59.6% homology to that from Lycopersicon esculentum. Northern blot analysis revealed that the gene for OsProT (OsProT) was expressed in all organs examined, comparatively strongly in leaf sheath and stem. Salt treatment did not induce expression of OsProT but strongly induced expression of the gene for Δ1-pyrroline-5-carboxylate synthetase (P5CS), a key enzyme in Pro biosynthesis. Southern blot analysis revealed that OsProT has a gene family. OsProT specifically transported l-Pro in a transport assay using Xenopus laevis oocytes.

Keywords: Oryza sativa L; ProT; Tissue specific expression; Xenopus laevis oocyte; Electrophysiological assay

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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