Journal Article

A Rapid and Efficient System of <i>Agrobacterium</i> Infection-Mediated Transient Gene Expression in Rice Oc Cells and Its Application for Analysis of the Expression and Antisense Suppression of Preprophytosulfokine, a Precursor of Phytosulfokine-α, Encoded by <i>OsPSK</i> Gene

Heping Yang, Akiko Morita, Yoshikatsu Matsubayashi, Kenzo Nakamura and Youji Sakagami

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 6, pages 811-816
Published in print June 2000 | ISSN: 0032-0781
Published online June 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/41.6.811
A Rapid and Efficient System of Agrobacterium Infection-Mediated Transient Gene Expression in Rice Oc Cells and Its Application for Analysis of the Expression and Antisense Suppression of Preprophytosulfokine, a Precursor of Phytosulfokine-α, Encoded by OsPSK Gene

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A rapid and efficient system for Agrobacterium infection-mediated transient gene expression in rice has been developed. Using this system, transient expression of preprophytosulfokine, a precursor of phytosulfokine-α, encoded by OsPSK gene was analyzed. The results suggest that the Agrobacterium infection-mediated transient gene expression system is as efficient in rice Oc cells as in tobacco BY-2 cells and might be useful for rapid analysis not only of foreign gene expression, but also of antisense gene suppression.

Keywords: Agrobacterium tumefaciens; Phytosulfokine-α; OsPSK; Sulfated protein; Transient gene expression

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Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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