Journal Article

Jasmonate Induction of Putrescine <i>N</i>-Methyltransferase Genes in the Root of <i>Nicotiana sylvestris</i>

Tsubasa Shoji, Yasuyuki Yamada and Takashi Hashimoto

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 7, pages 831-839
Published in print July 2000 | ISSN: 0032-0781
Published online July 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcd001
Jasmonate Induction of Putrescine N-Methyltransferase Genes in the Root of Nicotiana sylvestris

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Nicotine alkaloids are synthesized in the root of Nicotiana species, and their synthesis increases after insect attack, wounding and jasmonate treatment of the leaf. Putrescine N-methyltransferase (PMT) catalyzes the first committed step in nicotine biosynthesis. The expression patterns of the three Nicotiana sylvestris PMT genes (NsPMT1, NsPMT2, and NsPMT3) are reported in this study. Transcripts of the NsPMT genes were detected only in the root, and were up-regulated by methyl jasmonate treatment. When the 5′-flanking regions of NsPMT1, NsPMT2, and NsPMT3 were fused independently to β-glucuronidase reporter gene and introduced into N. sylvestris by Agrobacterium-mediated transformation, all introduced transgenes were expressed in the cortex, endodermis, and xylem in the root, as well as upregulated by methyl jasmonate treatment. These qualitatively similar patterns of expression for the NsPMT genes are achieved with only 0.25 kb of their conserved 5′-flanking regions, which contained no known jasmonate-responsive elements.

Keywords: Key words: Jasmonic acid — Nicotiana sylvestris — Nicotine — Putrescine N-methyltransferase.; Abbreviations: GUS, β-glucuronidase; MeJA, methyl jasmonate; NsPMT, Nicotiana sylvestris putrescine N-methyltransferase; PI, proteinase inhibitor; PMT, putrescine N-methyl-transferase.; The nucleotide sequences reported in this paper have been submitted to the DDBJ, EMBL and GenBank under accession numbers AB004322 (NsPMT1), AB004323 (NsPMT2) and AB004324 (NsPMT3).

Journal Article.  5915 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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