Journal Article

Mechanism of the Chilling-Induced Decrease in Proton Pumping across the Tonoplast of Rice Cells

Kunihiro Kasamo, Mineo Yamaguchi and Yoshiyuki Nakamura

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 7, pages 840-849
Published in print July 2000 | ISSN: 0032-0781
Published online July 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcd002
Mechanism of the Chilling-Induced Decrease in Proton Pumping across the Tonoplast of Rice Cells

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The ATP-generated proton pumping across tonoplast vesicles from chilling-sensitive Boro rice (Oryza sativa L. var. Boro) cultured cells was markedly decreased by chilling at 5°C for 3 d. The membrane fluidity of core hydrophobic and surface hydrophilic regions of the lipid bilayer was measured by steady-state fluorescence depolarization of 1,6-diphenyl-1,3,5-hexatriene and trimethylammonium 1,6-diphenyl-1,3,5-hexatriene and by electron spin resonance spectroscopy of 16- and 5-doxyl stearic acid, respectively. The fluidity of the surface region of the lipid bilayer of the tonoplast vesicles decreased by chilling. The fluidity of the surface region of the liposomes and the proton pumping across the reconstituted proteoliposomes with tonoplast H+-ATPase decreased with increasing content of the glycolipids. The proton pumping across chimera proteoliposomes was reduced by chilling only when it was reconstituted in the presence of tonoplast glycolipids from chilled Boro cells. These data suggest that the reduction in ATP-generated proton pumping across the tonoplast by chilling is due to the decrease in the fluidity of the surface region of the lipid bilayer of the tonoplast, which is caused by the changes in glycolipids.

Keywords: Key words: Chilling—Chimera proteoliposomes—Glycolipids—Membrane fluidity—Oryza sativa L.—Tonoplast H+-ATPase.; Abbreviations: DGDG, digalactosyldiacylglycerol; DOC, de-oxy-cholate; DOSA, doxyl stearic acid; DPH, 1,6-diphenyl-1,3,5-hexatriene; ESR, electron spin resonance; FCCP, carbonylcyanide-p-trifluoromethoxyphenyl hydrazone; n-OG, n-octylglucoside; TMA-DPH, trimethylammonium DPH.

Journal Article.  5660 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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