Journal Article

Demonstration of Cl<sup>–</sup> Requirement for Inhibition of Vacuolar Acidification by Cycloprodigiosin in Situ

Tsuyoshi Nakayasu, Keiko Kawauchi, Hajime Hirata and Teruo Shimmen

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 7, pages 857-863
Published in print July 2000 | ISSN: 0032-0781
Published online July 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcd004
Demonstration of Cl– Requirement for Inhibition of Vacuolar Acidification by Cycloprodigiosin in Situ

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Applying vacuole-perfusion and plasma membrane per-meabilization techniques to internodal cells of Chara, we analyzed the requirement of Cl for the action of cycloprodigiosin (cPrG) to inhibit vacuole acidification in situ. By combining the two techniques, the Cl concentration on both sides of the tonoplast could be controlled. In permeabilized cell fragments lacking Cl in the vacuole, the inhibitory effect of cPrG on vacuole acidification was cancelled. On the other hand, Cl in the cytoplasm was not needed for the cPrG action. These results supported the function of cPrG as a H+/Cl symporter. Requirement of Cl for the cPrG action was also demonstrated in vacuole-perfused living cells. This is the first report on the mechanism of cPrG action in situ.

Keywords: Key words: Characeae — Cl– — Cycloprodigiosin — Proton pump — Vacuole.; Abbreviations: ACS, artificial cell sap; APW, artificial pond water; V-ATPase, vacuolar-ATPase; cPrG, cycloprodigiosin; EGTA, ethyleneglycol-bis-(β-aminoethylether)N,N,N′N′-tetra-acetic acid; pHv, pH in the vacuole; V-PPase, vacuolar-pyrophosphatase; PPi, pyrophosphate; NR, neutral red.

Journal Article.  4027 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.