Journal Article

Characterization and Expression of Monosaccharide Transporters (<i>OsMSTs</i>) in Rice

Kyoko Toyofuku, Michihiro Kasahara and Junji Yamaguchi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 8, pages 940-947
Published in print August 2000 | ISSN: 0032-0781
Published online August 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcd016
Characterization and Expression of Monosaccharide Transporters (OsMSTs) in Rice

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This study deals with the cloning and characterization of monosaccharide transporter cDNAs in rice. OsMST1–3 (Oryza sativa monosaccharide transporters 1–3) have two sets of putative six transmembrane domains separated by a central long hydrophilic region. Heterologous expression of OsMST3 in the yeast Saccharomyces cerevisiae indicated that OsMST3 has transport activity for some monosaccharides in an energy-dependent H+ co-transport manner. Northern blot and in situ hybridization analyses showed that OsMST3 mRNA is detectable in leaf blades, leaf sheaths, calli and roots, especially the xylem as well as in sclerenchyma cells in the root. These results suggested that OsMST3 is involved in the accumulation of monosaccharides required for cell wall synthesis at the stage of cell thickening.

Keywords: Key words: Cell-specific expression — Energy-dependent active transport — Hexose transporter — Oryza sativa L. — Yeast expression.; Abbreviations: ABA, abscisic acid; CCCP, carbonyl-cyanide-m-chlorophenyl-hydrazone; 3-OMG, 3-O-methyl glucose.

Journal Article.  4530 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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