Journal Article

Regulation of Elongation Growth by Gibberellin in Root Segments of <i>Lemna</i> <i>minor</i>

Sayaka Inada and Teruo Shimmen

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 8, pages 932-939
Published in print August 2000 | ISSN: 0032-0781
Published online August 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcd018
Regulation of Elongation Growth by Gibberellin in Root Segments of Lemna minor

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Hormonal control of elongation growth was analyzed in segments excised from the elongation zone of Lemna roots. Exogenous GA3 did not promote the segment elongation but rather inhibited it. Uniconazole-P, a gibberellin biosynthesis inhibitor, significantly inhibited the segment elongation, and the inhibitory effect was completely nullified by GA3. In the epidermis, cell elongation was inhibited, but lateral cell expansion was not affected by uniconazole-P. Orientation of cortical microtubules of epidermal cells was disturbed by treatment with uniconazole-P for 12  h, and the disorganization of cortical microtubules was ameliorated by GA3. These findings suggested that disorganization of cortical microtubules induced inhibition of elongation growth of root. However, stabilization of cortical microtubules by taxol, a microtubule-stabilizing agent, did not affect the inhibition of segment elongation by uniconazole-P. These results suggested that endogenous gibberellin controls the elongation growth of root by regulating cell elongation.

Keywords: Key words: Cortical microtubule — Gibberellin — Lemna minor — Root elongation — Uniconazole-P — Taxol.; Abbreviations: CMT, cortical microtubule; DMSO, dimethylsulfoxide; EGTA, ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid; PBS, phosphate-buffered saline; PCMP, 2-(p-chlorophenoxy)-2-methylpropionic acid; PIPES, piperazine-N,N′-bis(2-ethanesulfonic acid); Un-P, uniconazole-P.

Journal Article.  4467 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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