Journal Article

A Novel Glycolate Oxidase Requiring Flavin Mononucleotide as the Cofactor in the Prasinophycean Alga <i>Mesostigma viride</i>

Koji Iwamoto and Tomoyoshi Ikawa

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 8, pages 988-991
Published in print August 2000 | ISSN: 0032-0781
Published online August 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcd020
A Novel Glycolate Oxidase Requiring Flavin Mononucleotide as the Cofactor in the Prasinophycean Alga Mesostigma viride

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A new type of glycolate-oxidizing enzyme was found in the prasinophycean alga Mesostigma viride. This enzyme was characterized as the glycolate oxidase that strongly requires FMN, thus distinguishing it from most other Prasinophytes that possess glycolate dehydrogenase instead.

Keywords: Key words: Flavin mononucleotide (FMN)-requiring enzyme — Glycolate metabolism — Glycolate oxidase — Glycolate-oxidizing enzyme — Mesostigma viride — Prasionophyceae.; Abbreviations: DCPIP, dichlorophenolindophenol; MLS root system, multilayered structure root system; p-CMB, p-chloromercuribenzoate; PHMS, 2-pyridylhydroxymethanesulfonate; PMSF, phenylmethylsulfonyl fluoride.

Journal Article.  2728 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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