Journal Article

Quantification of Ca<sup>2+</sup>/H<sup>+</sup> Antiporter VCAX1p in Vacuolar Membranes and Its Absence in Roots of Mung Bean

Hanayo Ueoka-Nakanishi and Masayoshi Maeshima

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 9, pages 1067-1071
Published in print September 2000 | ISSN: 0032-0781
Published online September 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcd023
Quantification of Ca2+/H+ Antiporter VCAX1p in Vacuolar Membranes and Its Absence in Roots of Mung Bean

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Vacuolar Ca2+/H+ antiporter VCAX1p, which contributes to the Ca2+ accumulation into vacuoles, was quantified by immunochemistry. The antiporter content in vacuolar membranes was 0.14 and 1.1 µg mg–1 of membrane protein for hypocotyls and epicotyls, respectively. The calculated turnover number was 120 s–1. Roots lacked the antiporter protein and the transcript.

Keywords: Key words: Ca2+/H+ antiporter — Organ specificity — Plant — Quantification — Specific activity — Vacuole.; Abbreviations: V-ATPase, vacuolar H+-ATPase; V-PPase, vacuolar H+-pyrophosphatase.

Journal Article.  3274 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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