Journal Article

Ethylene Suppresses Jasmonate-Induced Gene Expression in Nicotine Biosynthesis

Tsubasa Shoji, Keiji Nakajima and Takashi Hashimoto

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 9, pages 1072-1076
Published in print September 2000 | ISSN: 0032-0781
Published online September 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcd027
Ethylene Suppresses Jasmonate-Induced Gene Expression in Nicotine Biosynthesis

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In Nicotiana sylvestris, a set of nicotine biosynthesis genes were activated by exogenous application of methyl jasmonate, but the activation was effectively suppressed by simultaneous treatment with ethylene. When N. sylvestris transgenic hairy roots were treated with a natural ethylene precursor, the jasmonate-responsive expression of the promoter from a nicotine pathway enzyme gene was completely suppressed, and this suppressive effect was abolished when ethylene perception was blocked with silver cation. These and additional immunoblot results suggest that ethylene signal antagonizes jasmonate signal in nicotine biosynthesis.

Keywords: Key words: Ethylene — Jasmonate-Nicotiana sylvestris — Nicotine — Ornithine decarboxylase — Putrescine N-methyltransferase.; Abbreviations: ACC, 1-aminocyclopropane-1-carboxylic acid; GUS, β-glucuronidase; MeJA, methyl jasmonate; NsPMT, Nicotiana sylvestris putrescine N-methyltransferase; ODC, ornithine decarboxylase; PR-1b, pathogenesis-related protein I basic form; PI-II, proteinase inhibitor II; PMT, putrescine N-methyltransferase; SAMS, S-adenosylmethionine synthase.

Journal Article.  3360 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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