Journal Article

cDNA Cloning and Expression of an Aminoalcoholphosphotransferase Isoform in Chinese Cabbage

Young Hee Choi, Jin Kyung Lee, Choon-Hwan Lee and Sung Ho Cho

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 9, pages 1080-1084
Published in print September 2000 | ISSN: 0032-0781
Published online September 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcd030
cDNA Cloning and Expression of an Aminoalcoholphosphotransferase Isoform in Chinese Cabbage

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Aminoalcoholphosphotransferase (AAPT) catalyzes the synthesis of phosphatidylcholine and phosphatidylethanolamine from diacylglycerol plus a CDP-aminoalcohol such as CDP-choline or CDP-ethanolamine. Previously we reported the cloning of a cDNA encoding this enzyme from Chinese cabbage roots, and suggested the presence of possible isoforms [Min et al. (1997) J. Plant Biol. 40: 234]. We now report the cDNA cloning and expression analysis of a second AAPT from Chinese cabbage. This AAPT cDNA, AAPT2, contains an open reading frame of 1,170 bp coding for a protein of 389 amino acids. It shares 95% identity and 96% similarity with Chinese cabbage AAPT1 at the deduced amino acid level. The results from reverse transcriptase-PCR indicate that expression of AAPT2 is regulated temporally and up-regulated by low temperature.

Keywords: Key words: Aminoalcoholphosphotransferase — Chinese cabbage — Membrane — Phospholipid.; Abbreviations: AAPT, aminoalcoholphosphotransferase; PC, phosphatidylcholine; PE, phosphatidylethanolamine; RACE, rapid amplification of cDNA ends; RT-PCR, reverse transcriptase-PCR.; The nucleotide sequence reported in this paper has been submitted to the GenBank under accession number AF183933.

Journal Article.  2178 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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