Journal Article

<i>hosoba toge toge</i>, a Syndrome Caused by a Large Chromosomal Deletion Associated with a T-DNA Insertion in <i>Arabidopsis</i>

Hidetaka Kaya, Shusei Sato, Satoshi Tabata, Yasushi Kobayashi, Masaki Iwabuchi and Takashi Araki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 9, pages 1055-1066
Published in print September 2000 | ISSN: 0032-0781
Published online September 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcd032
hosoba toge toge, a Syndrome Caused by a Large Chromosomal Deletion Associated with a T-DNA Insertion in Arabidopsis

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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We isolated a T-DNA-tagged mutant named hosoba toge toge (hot) in which a pleiotropic phenotype was observed in both the shoot and root throughout the life cycle. The phenotype and allelism indicated that the mutant has a defect in both the FASCIATA1 (FAS1) gene and the FT gene located on the bottom arm of chromosome 1. Analysis of the junctions between the T-DNA ends and the plant genome suggested the presence of a 75.8-kbp deletion at the insertion site. In addition to FAS1 and FT, 13 genes were predicted to exist in the region corresponding to that deleted in hot. They include homologs of genes for type II inositol-1,4,5-triphosphate 5-phosphatase (IP5Pase), the β-chain of N-acetyl-β-glucosaminidase (NAGase), NADPH oxidoreductase of the ζ-crystallin family, polygalacturonase, and endo-1,4-β-glucanase. Although most aspects of the hot phenotype can be explained by loss of FAS1 and FT functions, some novel phenotypic features which may represent aspects of a mutant phenotype due to loss-of-function of other gene(s) were observed. One “wild-type” ecotype and a previously reported T-DNA insertion line, neither of which has any obvious phenotypic abnormality, carry a possible loss-of-function mutation in the ζ-crystallin homolog and in the NAGase β chain homolog, respectively.

Keywords: Key words: Arabidopsis — Chromosomal deletion — FASCIATA1-FT region —T-DNA insertion.; Abbreviations: ACC, aminocyclopropane-1-carboxylate; APH, aminoglycoside phosphotransferase; BAC, bacterial artificial chromosome; EST, expressed sequence tag; IP5Pase, inositol-1,4,5-triphosphate 5-phosphatase; LB, left border; MS medium, Murashige and Skoog’s medium; NAGase, N-acetyl-β-glucosaminidase; RB, right border; RT-PCR, reverse transcription PCR; SAM, shoot apical meristem; TAIL-PCR, thermal asymmetric interlaced PCR.

Journal Article.  7488 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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