Journal Article

Synthesis of Phosphatidylserine in Carrot Cells Cultured under Carbon-Source Starvation

Kumariah Manoharan, Hyun Sook Chae, Jae Myoung, Sung Ho Cho, Sung Ho Shin, Bong Heuy Cho and Woo Sung Lee

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 10, pages 1143-1148
Published in print October 2000 | ISSN: 0032-0781
Published online October 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcd041
Synthesis of Phosphatidylserine in Carrot Cells Cultured under Carbon-Source Starvation

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When carrot suspension cells were cultured on medium containing no carbon source (starvation), the levels of phosphatidylserine (PS) increased transiently 3–4 d after the initiation of starvation while levels of most other phospholipid (PL) species decreased. We previously reported that fatty acids of these PLs served as an alternative carbon source during starvation. The present study showed that cells possess two different biosynthetic pathways involving phosphatidylcholine (PC)/phosphatidylethanolamine (PE) exchange enzymes and PS synthase to synthesize PS. These activities peaked similarly 4 d after the initiation of starvation and coincided with the peak of PS level. The synthesis of serine was also significantly activated during starvation. The activity of phosphoserine aminotransferase (PSAT) which is involved in serine synthesis increased with a time course similar to that of the increase in the PS level. These observations suggest that the increase in PS level plays an important role in membranes which are degraded during starvation.

Keywords: Key words: Carbon starvation — Exchange reaction — Phosphatidylserine — PS synthase.; Abbreviations: CDP, cytidine 5-diphosphate; CMP, cytidine 5-monophosphate; DAG, diacylglyceride; Glc, glucose; PA, phosphatidic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PL, phospholipid; PLD, phospholipase D; PS, phosphatidylserine; PSAT, phosphoserine aminotransferase; TLC, thin-layer chromatography.

Journal Article.  4186 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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