Journal Article

An Agravitropic Mutant of Arabidopsis, <i>endodermal-amyloplast less 1</i>, That Lacks Amyloplasts in Hypocotyl Endodermal Cell Layer

Kenichiro Fujihira, Tetsuya Kurata, Masaaki K. Watahiki, Ichirou Karahara and Kotaro T. Yamamoto

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 11, pages 1193-1199
Published in print November 2000 | ISSN: 0032-0781
Published online November 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcd046
An Agravitropic Mutant of Arabidopsis, endodermal-amyloplast less 1, That Lacks Amyloplasts in Hypocotyl Endodermal Cell Layer

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We have isolated a new recessive mutant of Arabidopsis thaliana for gravitropism, endodermal-amyloplast less1 (eal1). eal1 shows reduced gravitropism in hypocotyl, and completely lacks gravitropism in inflorescence stems; root gravitropism is not affected. Starch staining with I-KI solution reveals almost no amyloplasts in eal1 hypocotyls when grown on a sucrose-free medium, though the root columella cells contain as many amyloplasts as wild type. On a medium containing 1% sucrose, eal1 hypocotyls contain as many starch granules as those of wild type, suggesting that starch synthesis is not affected in eal1. The endodermal cell layer which is thought to function as statocytes in hypocotyls is present in eal1. These results suggest that differentiation or development of gravity-responsive amyloplasts are affected in eal1 hypocotyls.

Keywords: Key words: Amyloplast — Arabidopsis thaliana — Endodermis — Gravitropic mutant.; Abbreviations: CAPS, co-dominant cleaved amplified polymorphic sequences; FAA, Formalin-acetic acid-alcohol; SSLP, simple sequence length polymorphisms.

Journal Article.  3998 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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