Journal Article

Involvement of Calcium-Dependent Protein Kinase in Rice (<i>Oryza sativa</i> L.) Lamina Inclination Caused by Brassinolide

Guangxiao Yang and Setsuko Komatsu

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 11, pages 1243-1250
Published in print November 2000 | ISSN: 0032-0781
Published online November 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcd050
Involvement of Calcium-Dependent Protein Kinase in Rice (Oryza sativa L.) Lamina Inclination Caused by Brassinolide

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  • Biochemistry
  • Molecular and Cell Biology
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Promotive effect of brassinolide (BL) on green lamina inclination was concentration-dependent when excised rice (Oryza sativa L.) lamina was floated on BL solution under continuous light conditions. Protein kinase inhibitor staurosporine and Ca2+ channel blocker LaCl3 could completely, while Ca2+ chelator EGTA could partially inhibit the lamina inclination caused by BL. Two protein kinases with apparent molecular masses of 45 and 54 kDa were detected using an in-gel kinase assay with histone III-S as a substrate. In particular, the changes in 45 kDa protein kinase activity correlated with lamina inclination caused by BL. The 45 kDa kinase activity was inhibited by Ca2+ chelator EGTA, protein kinase inhibitor, staurosporine and calmodulin antagonist W-7. Therefore, this 45 kDa protein kinase was identified as a Ca2+-dependent protein kinase (CDPK). Patterns of 2-dimensional PAGE after in vitro phosphorylation of crude extracts showed that the phosphorylation of 56 and 41 kDa proteins, which was Ca2+ -dependent, was strongly increased by BL treatment. These results suggested that CDPK and Ca2+-dependent protein phosphorylation are involved in BL-induced rice lamina inclination.

Keywords: Key words: Brassinolide — CDPK — Protein kinase — Protein phosphorylation — Rice (Oryza sativa L.) — Lamina inclination.; Abbreviations: BL, brassinolide; BR, brassinosteroid; CDPK, Ca2+-dependent protein kinase.

Journal Article.  4645 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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