Journal Article

Aromatic Monoamine-Induced Immediate Oxidative Burst Leading to an Increase in Cytosolic Ca<sup>2+</sup> Concentration in Tobacco Suspension Culture

Tomonori Kawano, Reinhard Pinontoan, Nobuyuki Uozumi, Chikahiro Miyake, Kozi Asada, Pappachan E. Kolattukudy and Shoshi Muto

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 11, pages 1251-1258
Published in print November 2000 | ISSN: 0032-0781
Published online November 2000 | e-ISSN: 1471-9053 | DOI:
Aromatic Monoamine-Induced Immediate Oxidative Burst Leading to an Increase in Cytosolic Ca2+ Concentration in Tobacco Suspension Culture

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry


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Aromatic monoamines may contribute to both chemical and physical protection of plants. Addition of phenylethylamine (PEA) and benzylamine to tobacco suspension culture (cell line BY-2) induced a very rapid and transient generation of two active oxygen species (AOS), H2O2 and superoxide anion, both detected with chemiluminescence. Electron spin resonance spectroscopy revealed that hydroxy radicals are also produced. With laser-scanning confocal microscopy, fluorescence spectroscopy and microplate fluorescence reading, intracellular H2O2 production was detected using dichlorofluorescin diacetate as a fluorescent probe. Following AOS production, cytosolic Ca2+ concentration ([Ca2+]c) of the tobacco cells, monitored with luminescence of transgenic aequorin, increased and attained to a peak level 12 s after PEA addition. The PEA-induced increase in [Ca2+]c was inhibited by a Ca2+ chelator, Ca2+ antagonists and AOS scavengers, suggesting that PEA-induced AOS triggered a Ca2+ influx across the plasma membrane.

Keywords: Key words: Active oxygen species — Calcium — Electron spin resonance — Monoamine — Nicotiana tabacum — Oxidative burst.; Abbreviations: AMAs, aromatic monoamines and their derivatives; AOS, active oxygen species; BAPTA, 1,2-bis(2-aminophenoxy)-ethane-N,N,N′,N′-tetraacetic acid; BAPTA-AM, BAPTA acetoxymethyl ester, bipy, 2,2′-bipyridyl; [Ca2+]c, cytosolic free Ca2+ concentration; CL, chemiluminescence; CLA, 2-methyl-6-phenyl-3,7-dihydroimidazo[1,2-a]pyrazin-3-one; DAPI, 4,6-diamidino-2-phenylindole; DCF, dichlorofluorescein; DCFH, dichlorofluorescin; DCFH-DA, dichlorofluorescin diacetate; DMPO, 5,5′-di-methyl-1-pirroline-N-oxide; DMTU, N,N′-dimethylthiourea; ESR, electron spin resonance; HO., hydroxyl radical; O2.–, superoxide anion; o-phe, 1,10-phenanthroline; PEA, phenylethylamine; rlu, relative luminescence units.

Journal Article.  5219 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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