Journal Article

Identification of Porin-Like Polypeptide(s) in the Boundary Membrane of Oilseed Glyoxysomes

F. Javier Corpas, Luisa M. Sandalio, Michael J. Brown, Luis A. del Río and Richard N. Trelease

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 11, pages 1218-1228
Published in print November 2000 | ISSN: 0032-0781
Published online November 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcd054
Identification of Porin-Like Polypeptide(s) in the Boundary Membrane of Oilseed Glyoxysomes

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A 36-kDa polypeptide of unknown function was identified by us in the boundary membrane fraction of cucumber seedling glyoxysomes. Evidence is presented in this study that this 36-kDa polypeptide is a glyoxysomal membrane porin. A sequence of 24 amino acid residues derived from a CNBr-cleaved fragment of the 36-kDa polypeptide revealed 72% to 95% identities with sequences in mitochondrial or non-green plastid porins of several different plant species. Immunological evidence indicated that the 36-kDa (and possibly a 34-kDa polypeptide) was a porin(s). Antiserum raised against a potato tuber mitochondrial porin recognized on immunoblots 34-kDa and 36-kDa polypeptides in detergent-solubilized membrane fractions of cucumber seedling glyoxysomes and mitochondria, and in similar glyoxysomal fractions of cotton, castor bean, and sunflower seedlings. The 36-kDa polypeptide seems to be a constitutive component because it was detected also in membrane protein fractions derived from cucumber leaf-type peroxisomes. Compelling evidence that one or both of these polypeptides were authentic glyoxysomal membrane porins was obtained from electron microscopic immunogold analyses. Antiporin IgGs recognized antigen(s) in outer membranes of glyoxysomes and mitochondria. Taken together, the data indicate that membranes of cucumber (and other oilseed) glyoxysomes, leaf-type peroxisomes, and mitochondria possess similar molecular mass porin polypeptide(s) (34 and 36 kDa) with overlapping immunological and amino acid sequence similarities.

Keywords: Key words: Cucumber (Cucumis sativa) — Glyoxysomes — Immunogold — Peroxisomal membrane protein — Peroxisome — Porin.; Abbreviations: APX, ascorbate peroxidase; CCO, cytochrome c oxidase; CNBr, cyanogen bromide; PMP, peroxisomal membrane protein; SDS-PAGE, sodium dodecylsulfate polyacrylamide gel electrophoresis.

Journal Article.  7720 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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