Journal Article

Molecular Characterization of cDNA Encoding Oxygen Evolving Enhancer Protein 1 Increased by Salt Treatment in the Mangrove <i>Bruguiera gymnorrhiza</i>

Koichi Sugihara, Nobutaka Hanagata, Zvy Dubinsky, Sigeyuki Baba and Isao Karube

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 11, pages 1279-1285
Published in print November 2000 | ISSN: 0032-0781
Published online November 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcd061
Molecular Characterization of cDNA Encoding Oxygen Evolving Enhancer Protein 1 Increased by Salt Treatment in the Mangrove Bruguiera gymnorrhiza

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Young plants of the common Okinawa mangrove species Bruguiera gymnorrhiza were transferred from freshwater to a medium with seawater salt level (500 mM NaCl). Two-dimensional gel electrophoresis revealed in the leaf extract of the plant a 33 kDa protein with pI 5.2, whose quantity increased as a result of NaCl treatment. The N-terminal amino acids sequence of this protein had a significant homology with mature region of oxygen evolving enhancer protein 1 (OEE1) precursor. The cloning of OEE1 precursor cDNA fragment was carried out by means of reverse transcription-PCR (RT-PCR) using degenerated primers. Both 3′- and 5′-regions were isolated by rapid amplification of cDNA ends (RACE) method. The deduced amino acid sequence consisted of 322 amino acids and was 87% identical to that of Nicotiana tabacum. In B. gymnorrhiza, the predicted amino acid sequence of the mature protein starts at the residue number 85 of the open reading frame. The first 84-amino acid residues correspond to a typical transit sequence for the signal directing OEE1 to its appropriate compartment of chloroplast. The expression of OEE1 was analyzed together with other OEE subunits and D1 protein of photosystem II. The transcript levels of all the three OEEs were enhanced by NaCl treatment, but the significant increase of D1 protein was not observed.

Keywords: Key words: Bruguiera gymnorrhiza — Molecular cloning — Oxygen evolving enhancer protein 1 — Salt tolerance — Two-dimensional gel electrophoresis.

Journal Article.  4165 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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