Journal Article

Distribution of Expansins in Graviresponding Maize Roots

Nenggang Zhang and Karl H. Hasenstein

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 12, pages 1305-1312
Published in print December 2000 | ISSN: 0032-0781
Published online December 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcd064
Distribution of Expansins in Graviresponding Maize Roots

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To test if expansins, wall loosening proteins that disrupt binding between microfibrils and cell wall matrix, participate in the differential elongation of graviresponding roots, Zea mays L. cv. Merit roots were gravistimulated and used for immunolocalization with anti-expansin. Western blots showed cross-reaction with two proteins of maize, one of the same mass as cucumber expansin (29 kDa), the second slightly larger (32 kDa). Maize roots contained mainly the larger protein, but both were found in coleoptiles. The expansin distribution in cucumber roots and hypocotyls was similar to the distribution in maize. Roots showed stronger expansin signals on the expanding convex side than the concave flank as early as 30 min after gravistimulation. Treatment with brefeldin A, a vesicle transport inhibitor, or the auxin transport inhibitor, naphthylphthalamic acid, showed delayed graviresponse and the appearance of differential staining. Our results indicate that expansins may be transported and secreted to cell walls via vesicles and function in wall expansion.

Keywords: Key words: Expansin — Graviresponse — Immunolocalization — Zea mays L.; Abbreviations: BFA, brefeldin A; NPA, naphthylphthalamic acid; QC, quiescent center.

Journal Article.  4061 words.  Illustrated.

Subjects: Plant Sciences and Forestry ; Biochemistry ; Molecular and Cell Biology

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