Journal Article

Resistance Induction in Barley Coleoptile Cells by Intracellular pH Decline

Naoto Yamaoka, Shizuo Yoshida, Erika Motoyama, Yoji Takeuchi, Yasuhiro Takada and Noriyuki Fukunaga

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 12, pages 1321-1326
Published in print December 2000 | ISSN: 0032-0781
Published online December 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcd065
Resistance Induction in Barley Coleoptile Cells by Intracellular pH Decline

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Cytoplasmic acidification in suspension-cultured plant cells has been characterized as a common intracellular response of some kinds of plant cells to elicitors. Expression of various defense genes in these cells has been increased by the cytoplasmic acidification itself without treatment by elicitors. It is not evident, however, whether or not cells with acidified cytoplasm actually exhibit resistance to the pathogen because of the lack of an adequate infection system between cultured plant cells and some pathogens. Using barley coleoptiles rather than suspension-cultured cells, we demonstrated both detection of cellular pH decline and increased resistance to Blumeria graminis. The cytoplasmic pH of barley coleoptile cells floated on 1 mM citrate buffer (CB), pH 4.0, became 0.5 unit lower than that of cells floated on 1 mM CB, pH 8.0, within 30 min after treatment. The penetration efficiency of B. graminis into the coleoptile was decreased in a pH-dependent manner; that is, when the coleoptiles were floated on 1 mM CB, pH 8.0, the penetration efficiency of the fungi was about 80%. In contrast, when the coleoptiles were floated on acidic buffers, the penetration efficiency decreased in parallel the decline of pH and the penetration efficiency reached 0% when coleoptiles were floated on 1 mM CB, pH 4.0. Morphogenesis of appressoria on the coleoptiles floating on CB was not influenced. The lowered penetration efficiency at lower pH was partially cancelled when the barley coleoptiles were irradiated with UV for 5 min prior to B. graminis inoculation. These findings suggest that the decline in cytoplasmic pH in barley coleoptile cells increases resistance to the pathogenic fungus B. graminis.

Keywords: Key words: Blumeria graminisf. sp. hordei — Cytoplasmic acidification —Hordeum vulgare — Resistance induction.; Abbreviations: CB, citrate buffer; FDA, fluorescein diacetate; HR, hypersensitive reaction.

Journal Article.  3113 words.  Illustrated.

Subjects: Plant Sciences and Forestry ; Biochemistry ; Molecular and Cell Biology

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