Journal Article

Isolation and Purification of the Reaction Center (RC) and the Core (RC-LH1) Complex from <i>Rhodobium marinum</i>: the LH1 Ring of the Detergent-Solubilized Core Complex Contains 32 Bacteriochlorophylls

Pu Qian, Tatsuo Yagura, Yasushi Koyama and Richard J. Cogdell

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 12, pages 1347-1353
Published in print December 2000 | ISSN: 0032-0781
Published online December 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcd068
Isolation and Purification of the Reaction Center (RC) and the Core (RC-LH1) Complex from Rhodobium marinum: the LH1 Ring of the Detergent-Solubilized Core Complex Contains 32 Bacteriochlorophylls

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The reaction center (RC) and the core (RC-LH1) complex were isolated and purified from Rhodobium marinum; together with the LH1 complex [Meckenstock et al. (1992a) FEBS Lett. 311: 128], a complete set of RC, LH1 and RC-LH1 from the same wild-type strain of a purple photosynthetic bacterium can therefore now be made. Comparison of the BChl a/BPhe a ratio (determined by HPLC) between the RC and the RC-LH1 complexes lead us to the determination of the number of BChls in the LH1 ring to be 32.06±2.90, indicating that the LH1 ring from Rh. marinum consists of 16 αβ subunits.

Keywords: Key words: LH1 antenna complex — Number of αβ subunits — Reaction center — Reaction center-LH1 core complex —Rhodobium marinum.; Abbreviations: BChl, bacteriochlorophyll; BPhe, bacteriopheophytin; Car, carotenoid; DSM, Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH; LDAO, lauryl-N,N-dimethyl-amine-N-oxide; RC, reaction center; LH1, light harvesting complex 1.

Journal Article.  4416 words.  Illustrated.

Subjects: Plant Sciences and Forestry ; Biochemistry ; Molecular and Cell Biology

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