Journal Article

Evidence for the Involvement of Phospholipase C in the Anaerobic Signal Transduction

Remo Reggiani and Paolo Laoreti

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 41, issue 12, pages 1392-1396
Published in print December 2000 | ISSN: 0032-0781
Published online December 2000 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pcd073
Evidence for the Involvement of Phospholipase C in the Anaerobic Signal Transduction

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Pre-treatment of rice roots for 2 h in aerobic conditions with two phospholipase C (PLC) antagonists, neomycin and compound 48/80 (C48/80), inhibited accumulation ofγ -aminobutyric acid and increased the loss of K+ in the medium during 3 h of anoxia. The presence of Ca2+ and A23187 (Ca2+ ionophore) nullified the effect of PLC inhibitors. Pre-treatment of rice roots with neomycin and C48/80 abolished the anaerobic increase in the concentration of the PLC product inositol 1,4,5-triphosphate. Stimulation of the anaerobic signal transduction pathway with aluminium fluoride (G protein activator) was attenuated by PLC inhibitors. These findings are consistent with the participation of PLC in the anaerobic response.

Keywords: Key words: γ-Aminobutyric acid — Anoxia — Inositol 1,4,5-triphosphate — Oryza sativa — Phospholipase C— Signal transduction.; Abbreviations: AlF4–, aluminium fluoride; CaM, calmodulin; C48/80, compound 48/80; GABA,γ -aminobutyric acid; GAD, glutamic acid decarboxylase; IP3, inositol 1,4,5-triphosphate; PLC, phospholipase C; RR, ruthenium red; TEA, tetraethylammonium chloride; TFP, trifluoperazine.

Journal Article.  2964 words.  Illustrated.

Subjects: Plant Sciences and Forestry ; Biochemistry ; Molecular and Cell Biology

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