Journal Article

Inhibition of Proteasome Activity by the TED4 Protein inExtracellular Space: a Novel Mechanism for Protection of Living Cells fromInjury Caused by DyingCells

Satoshi Endo, Taku Demura and Hiroo Fukuda

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 1, pages 9-19
Published in print January 2001 | ISSN: 0032-0781
Published online January 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce002
Inhibition of Proteasome Activity by the TED4 Protein inExtracellular Space: a Novel Mechanism for Protection of Living Cells fromInjury Caused by DyingCells

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In maturation process of tracheary element (TE) differentiation, many hydrolases are activated to execute programmed cell death of TEs. Such hydrolases are released from maturing TEs into extracellular space. The release of hydrolases should be harmful to surrounding cells. The TED4 protein, a tentative plant non-specific lipid transfer protein that is expressed preferentially in TE-induced culture of zinnia (Zinnia elegans L.), is secreted into the apoplastic space prior to and associated with morphological changes of TEs. Our studies on the interrelationship between the TED4 protein and proteolytic activities using an in vitro TE differentiation system of zinnia revealed the following facts. (1) Active proteasome is released into medium at maturation stage of TE differentiation. (2) The TED4 protein forms a complex with proteasome in culture medium. (3) The TED4 protein inhibits proteasome activity in the medium and crude extracts of zinnia cells. (4) The depletion of the TED4 protein from culture medium results in an increase in mortality of other living cells. These results strongly suggest that the secreted TED4 protein acts as an inhibitor of proteasome to protect other cells from undesirable injury due to proteolytic activities exudated from dying TEs.

Keywords: Key words: Lipid transfer protein — Programmed cell death — Protease inhibitor — Proteasome — Tracheary element differentiation — Zinnia elegans.; Abbreviations: Boc, t-butyloxycarbonyl; MCA, 4-methyl-coumaryl-7-amido; nsLTP, non-specific lipid transfer protein; PCD, programmed cell death; Suc, succinyl; TE, tracheary element; Z, carbobenzoxy.

Journal Article.  6765 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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