Journal Article

Biochemical Characterization of Profilin from Seeds of<i>Phaseolus vulgaris</i>L.

Gabriel Guillén, LorenaM.L. López-Sánchez, CarolinaSan Román-Roque, Federico Sánchez and MarcoA. Villanueva

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 1, pages 54-62
Published in print January 2001 | ISSN: 0032-0781
Published online January 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce006
Biochemical Characterization of Profilin from Seeds ofPhaseolus vulgarisL.

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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The isoform composition of the 14.4 kDa profilin polypeptide was analyzed in seeds, leaves, flowers, roots and root-nodules from Phaseolus vulgaris L. Isoforms of pIs ~4.4–5 were present in all the tissues analyzed. The biochemical features of the protein present in seed tissue were determined. Seed profilin bound to Phenyl-Sepharose under low salt conditions which suggested a hydrophobic interaction; however, it was not associated with microsomal membranes nor it partitioned as a hydrophobic protein in Triton X-114. Fractions eluting from poly-l-proline or Phenyl-Sepharose columns contained well detectable amounts of profilin but no actin, suggesting that most of the protein was not present as profilactin in the seed. However, seed profilin appeared to be in some kind of complex since several molecular weight species were observed on native gels. In addition, profilin was found preferentially in the embryo axis and light microscopic immunolocalization showed a cytoplasmic distribution in this tissue.

Keywords: Key words: Cytoskeleton — Isoforms — Profilin — Seeds — Phaseolus vulgaris.; Abbreviations: PBS, phosphate-buffered saline; PIP2, phosphatidylinositol-4,5-bisphosphate; DTT, dithiothreitol.

Journal Article.  6856 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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