Journal Article

An Arabinogalactan Protein(s) is a Key Component of a Fraction that Mediates Local Intercellular Communication Involved in Tracheary Element Differentiation of Zinnia Mesophyll Cells

Hiroyasu Motose, Munetaka Sugiyama and Hiroo Fukuda

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 2, pages 129-137
Published in print February 2001 | ISSN: 0032-0781
Published online February 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce014
An Arabinogalactan Protein(s) is a Key Component of a Fraction that Mediates Local Intercellular Communication Involved in Tracheary Element Differentiation of Zinnia Mesophyll Cells

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Local intercellular communication is involved in tracheary element (TE) differentiation of zinnia (Zinnia elegans L.) mesophyll cells and mediated by a proteinous macromolecule, which was designated xylogen. To characterize and isolate xylogen, a bioassay system to monitor the activity of xylogen was developed, in which mesophyll cells were embedded in microbeads of agarose gel at a low (2.0–4.3×104 cells ml–1) or high density (8.0–9.0×104 cells ml–1) and microbeads of different cell densities were cultured together in a liquid medium to give a total density of 2.1–2.5×104 cells ml–1. Without any additives, the frequency of TE differentiation was much smaller in the low-density microbeads than in the high-density microbeads. This low level of TE differentiation in the low-density microbeads was attributable to the shortage of xylogen. When cultures were supplemented with conditioned medium (CM) prepared from zinnia cell suspensions undergoing TE differentiation, the frequency of TE differentiation in the low-density microbeads increased remarkably, indicating the activity of xylogen in the CM. The xylogen activity in CM was sensitive to proteinase treatments. Xylogen was bound to galactose-specific lectins such as Ricinus communis agglutinin and peanut agglutinin, and precipitated by β-glucosyl Yariv reagent. These results indicate that xylogen is a kind of arabinogalactan protein.

Keywords: Key words: Arabinogalactan protein — Local intercellular communication — Tracheary element differentiation — Xylogen — Zinnia elegans.; Abbreviations: AGP, arabinogalactan protein; βGlcY, β-glucosyl Yariv reagent; Con A, concanavalin A; CM, conditioned medium; HMWF, high-molecular-weight fraction; LMWF, low-molecular-weight fraction; PNA, peanut agglutinin; PHA, Phaseolus vulgaris agglutinin; RCA, Ricinus communis agglutinin; TE, tracheary element; UEA, Ulex europaeus agglutinin; WGA, wheat germ agglutinin.

Journal Article.  6095 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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