Journal Article

Physical Arrangement of Retrotransposon-Related Repeats in Centromeric Regions of Wheat

Kazu-Nori Fukui, Go Suzuki, Evans S. Lagudah, Sadequr Rahman, Rudi Appels, Maki Yamamoto and Yasuhiko Mukai

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 2, pages 189-196
Published in print February 2001 | ISSN: 0032-0781
Published online February 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce026
Physical Arrangement of Retrotransposon-Related Repeats in Centromeric Regions of Wheat

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  • Biochemistry
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Cereal centromeres commonly contain many repetitive sequences that are derived from Ty3/gypsy retrotransposon. FISH analysis using a large DNA insert library of wheat identified a 67-kb clone (R11H) that showed strong hybridization signals on the centromeres. The R11H clone contains Ty3/gypsy retrotransposon-related sequences; both integrase and CCS1 family sequences were identified. Subsequently, we isolated additional 23 large-insert clones which also contained the integrase and CCS1 sequences. Based on the number of the integrase repeats in the clones determined by DNA gel blot analysis, we concluded that the retrotransposon-like sequences are tandemly repeated in wheat centromeres in ca. 55-kb interval on average. This conclusion is consistent with the results of FISH analysis on the extended DNA fibers.

Keywords: Key words: Centromere — Fluorescence in situ hybridization (FISH) — Large-insert library — Retrotransposon — Wheat.; Abbreviations: dig, digoxigenin; FISH, fluorescence in situ hybridization; LTR, long-terminal repeat; PCR, polymerase chain reaction; PI, propidium iodide.

Journal Article.  4128 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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