Journal Article

Hyperosmotic Stress Induces a Rapid and Transient Increase in Inositol 1,4,5-Trisphosphate Independent of Abscisic Acid in Arabidopsis Cell Culture

Seiji Takahashi, Takeshi Katagiri, Takashi Hirayama, Kazuko Yamaguchi-Shinozaki and Kazuo Shinozaki

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 2, pages 214-222
Published in print February 2001 | ISSN: 0032-0781
Published online February 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce028
Hyperosmotic Stress Induces a Rapid and Transient Increase in Inositol  1,4,5-Trisphosphate Independent of Abscisic Acid in Arabidopsis Cell Culture

More Like This

Show all results sharing these subjects:

  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

GO

Show Summary Details

Preview

Phospholipid metabolism is involved in hyperosmotic-stress responses in plants. To investigate the role of phosphoinositide-specific phospholipase C (PI-PLC)—a key enzyme in phosphoinositide turnover—in hyperosmotic-stress signaling, we analyzed changes in inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) content in response to hyperosmotic shock or salinity in Arabidopsis thaliana T87 cultured cells. Within a few s, a hyperosmotic shock, caused by mannitol, NaCl, or dehydration, induced a rapid and transient increase in Ins(1,4,5)P3. However, no transient increase was detected in cells treated with ABA. Neomycin and U73122, inhibitors of PI-PLC, inhibited the increase in Ins(1,4,5)P3 caused by the hyperosmotic shock. A rapid increase in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) in response to the hyperosmotic shock also occurred, but the rate of increase was much slower than that of Ins(1,4,5)P3. These findings indicate that the transient Ins(1,4,5)P3 production was due to the activation of PI-PLC in response to hyperosmotic stress. PI-PLC inhibitors also inhibited hyperosmotic stress-responsive expression of some dehydration-inducible genes, such as rd29A (lti78/cor78) and rd17 (cor47), that are controlled by the DRE/CRT cis-acting element but did not inhibit hyperosmotic stress-responsive expression of ABA-inducible genes, such as rd20. Taken together, these results suggest the involvement of PI-PLC and Ins(1,4,5)P3 in an ABA-independent hyperosmotic-stress signal transduction pathway in higher plants.

Keywords: Key words: Arabidopsis thaliana — Hyperosmotic stress — Inositol 1,4,5-trisphosphate — Phosphoinositide-specific phospholipase C — Signal transduction.; Abbreviations: CRT, C-repeat; DAG, diacylglycerol; DRE, dehydration-responsive element; EGTA, ethyleneglycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid; Ins(1,4,5)P3, inositol 1,4,5-trisphosphate; PCA, perchloric acid; PI-turnover, phosphoinositide-turnover; PIP, phosphatidylinositol monophosphate; PIP2, phosphatidylinositol bisphosphate; PI-PLC, phosphoinositide-specific phospholipase C; PtdIns(3,5)P2, phosphatidylinositol 3,5-bisphosphate; PtdIns(4,5)P2, phosphatidylinositol 4,5-bisphosphate; PtdIns4P-5-kinase, phosphatidylinositol-4-phosphate 5-kinase.

Journal Article.  6025 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.