Journal Article

The Chloroplast <i>clpP</i> Gene, Encoding a Proteolytic Subunit of ATP-Dependent Protease, is Indispensable for Chloroplast Development in Tobacco

Toshiharu Shikanai, Katsumi Shimizu, Katsumi Ueda, Yoshiki Nishimura, Tsuneyoshi Kuroiwa and Takashi Hashimoto

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 3, pages 264-273
Published in print March 2001 | ISSN: 0032-0781
Published online March 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce031
The Chloroplast clpP Gene, Encoding a Proteolytic Subunit
of ATP-Dependent Protease, is Indispensable for Chloroplast Development in
Tobacco

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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ClpP is a proteolytic subunit of the ATP-dependent Clp protease, which is found in chloroplasts in higher plants. Proteolytic subunits are encoded both by the chloroplast gene, clpP, and a nuclear multi gene family. We insertionally disrupted clpP by chloroplast transformation in tobacco. However, complete segregation was impossible, indicating that the chloroplast-encoded clpP gene has an indispensable function for cell survival. In the heteroplasmic clpP disruptant, the leaf surface was rough by clumping, and the lateral leaf expansion was irregularly arrested, which led to an asymmetric, slender leaf shape. Chloroplasts consisted of two populations: chloroplasts that were similar to the wild type, and small chloroplasts that emitted high chl fluorescence. Ultrastructural analysis of chloroplast development suggested that clpP disruption also induced swelling of the thylakoid lumen in the meristem plastids and inhibition of etioplast development in the dark. In mature leaves, thylakoid membranes of the smaller chloroplast population consisted exclusively of large stacks of tightly appressed membranes. These results indicate that chloroplast-encoded ClpP is involved in multiple processes of chloroplast development, including a housekeeping function that is indispensable for cell survival.

Keywords: Key words: ATP-dependent protease— Chloroplast development — Chloroplast transformation — Clp protease — Gene disruption — Thylakoid membrane — Tobacco (Nicotiana tabacum).; Abbreviation: MS medium, Murashige-Skoog medium.

Journal Article.  6266 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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