Journal Article

Molecular Cloning and Characterization of a cDNA for a Novel Ethylene Receptor, NT-ERS1, of Tobacco (<i>Nicotiana tabacum</i> L.)

Yoshifumi Terajima, Hideki Nukui, Akie Kobayashi, Shin Fujimoto, Shuu Hase, Toshihito Yoshioka, Teruyoshi Hashiba and Shigeru Satoh

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 3, pages 308-313
Published in print March 2001 | ISSN: 0032-0781
Published online March 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce038
Molecular Cloning and Characterization of a cDNA for a Novel Ethylene Receptor, NT-ERS1, of Tobacco (Nicotiana tabacum L.)

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The cDNA encoding a novel member (NT-ERS1) of ethylene receptor family of tobacco (Nicotiana tabacum L.) was obtained by a combination of RT-PCR and 5′-/3′-RACE cloning. The cDNA was 2,092 nucleotides long and had an open reading frame of 1,911 bp encoding 637 amino acids. The deduced polypeptide lacked a response regulator domain, indicating that the ethylene receptor belongs to an ERS-group. The amino acid sequence was similar to respective members of the tobacco ethylene receptor family: 67.8% to NT-ETR1, 39.1% to NTHK1 and 31.1% to NTHK2. Comparison of amino acid sequence suggested that NT-ERS1 is the counterpart of Nr in the ethylene receptor family of tomato, which belongs to Solanaceae as does tobacco. Northern blot analysis showed that mRNA of NT-ERS1 was present in leaf, shoot and root tissues, and accumulated in leaves treated with exogenous ethylene. A mutated NT-ERS1 cDNA transgene, obtained by introducing one nucleotide substitution into NT-ETR1 cDNA, conferred ethylene insensitivity in tobacco plants, indicating that the translation product of the cDNA actually functioned in the plants.

Keywords: Key words: Ethylene receptor genes — Ethylene-insensitive transgenic tobacco — Nicotiana tabacum — Solanaceae — Tobacco.; Abbreviations: ACC, 1-aminocyclopropane-1-carboxylate; CaMV, cauliflower mosaic virus; CMV-Y, cucumber mosaic virus yellow strain; MOPS, 4-morpholinopropanesulfonate; RACE, rapid amplification of cDNA ends; RT-PCR, reverse transcription-polymerase chain reaction.

Journal Article.  3960 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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