Journal Article

Mapping the Virus and Host Genes Involved in the Resistance Response in Cucumber Mosaic Virus-Infected <i>Arabidopsis thaliana</i>

Hideki Takahashi, Masashi Suzuki, Keiko Natsuaki, Takuma Shigyo, Kumiko Hino, Tohru Teraoka, Daijiro Hosokawa and Yoshio Ehara

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 3, pages 340-347
Published in print March 2001 | ISSN: 0032-0781
Published online March 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce039
Mapping the Virus and Host Genes Involved in the Resistance Response in Cucumber Mosaic Virus-Infected Arabidopsis thaliana

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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A yellow strain of cucumber mosaic virus (CMV) [CMV(Y)] induces a resistance response characterized by inhibition of virus systemic movement with development of necrotic local lesions in the virus-inoculated leaves of Arabidopsis thaliana ecotype C24. In this report, the avirulence determinant in the virus genome was defined and the resistance gene (RCY1) of C24 was genetically mapped. The response of C24 to CMV containing the chimeric RNA3 between CMV(Y) and a virulent strain of CMV indicated that the coat protein gene of CMV(Y) determined the localization of the virus in the inoculated leaves of C24. The RCY1 locus was mapped between two CAPS markers, DFR and T43968, which were located in the region containing genetically defined disease resistance genes and their homologues. These results indicate that the resistance response to CMV(Y) in C24 is determined by the combination of the coat protein gene and RCY1 on chromosome 5.

Keywords: Key words: Avirulence gene — Coat protein — Cucumber mosaic virus — Gene-for-gene — Hypersensitive response — Resistance gene.; Abbreviations: CAPS, cleaved amplified polymorphic sequence; ch., chromosome; Col, Columbia; CMV, cucumber mosaic virus; HR, hypersensitive response; NLLs, necrotic local lesions; LRR, leucine-rich repeat; PR, pathogenesis-related; PVX, potato virus X; R-ESTs, sequence tags showing similarity to disease resistance genes; RT, reverse transcription; SA, salicylic acid; SSLP, simple sequence length polymorphisms; TCV, turnip crinkle virus; TMV, tobacco mosaic virus.

Journal Article.  5183 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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