Journal Article

Role of the Putative Membrane-Bound Endo-1,4-β-Glucanase KORRIGAN in Cell Elongation and Cellulose Synthesis in <i>Arabidopsis thaliana</i>

Shigeru Sato, Tomohiko Kato, Koichi Kakegawa, Tadashi Ishii, Yao-Guang Liu, Tatsuya Awano, Keiji Takabe, Yoshiharu Nishiyama, Shigenori Kuga, Shusei Sato, Yasukazu Nakamura, Satoshi Tabata and Daisuke Shibata

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 3, pages 251-263
Published in print March 2001 | ISSN: 0032-0781
Published online March 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce045
Role of the Putative Membrane-Bound Endo-1,4-β-Glucanase KORRIGAN in Cell Elongation and Cellulose Synthesis in Arabidopsis thaliana

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A temperature-sensitive, elongation-deficient mutant of Arabidopsis thaliana was isolated. At the non-permissive temperature of 31°C, the mutation impaired tissue elongation; otherwise, tissue development was normal. Hypocotyl cells that had established cell walls at 21°C under light-dark cycles ceased elongation and swelled when the mutant was shifted to 31°C and darkness, indicating that the affected gene is essential for cell elongation. Analysis of the cell walls of mutant plants grown at 31°C revealed that the cellulose content was reduced to 40% and the pectin content was increased to 162% of the corresponding values for the wild type grown at the same temperature. The increased amounts of pectin in the mutant were bound tightly to cellulose microfibrils. No change in the content of hemicellulose was apparent in the 31°C-adapted mutant. Field emission–scanning electron microscopy suggested that the structure of cellulose bundles was affected by the mutation; X-ray diffraction, however, revealed no change in the crystallite size of cellulose microfibrils. The regeneration of cellulose microfibrils from naked mutant protoplasts was substantially delayed at 31°C. The recessive mutation was mapped to chromosome V, and map-based cloning identified it as a single G→A transition (resulting in a Gly429→Arg substitution) in KORRIGAN, which encodes a putative membrane-bound endo-1,4-β-glucanase. These results demonstrate that the product of this gene is required for cellulose synthesis.

Keywords: Key words: Arabidopsis thaliana — Cell elongation — Cell wall — Cellulose synthesis — Membrane-bound endo-1,4-β-glucanase — Temperature-sensitive mutant.; Abbreviations: CAPS, cleaved amplified polymorphic sequences; DCB, 2,6-dichlorobenzonitrile; FE-SEM, field emission–scanning electron microscopy; SSLP, simple sequence length polymorphisms; TAC, transformation-competent artificial chromosome; TEM, transmission electron microscopy; TFA, trifluoroacetic acid.

Journal Article.  8431 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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