Journal Article

A <i>Brassica oleracea</i> Gene Expressed in a Variety-Specific Manner May Encode a Novel Plant Transmembrane Receptor

Joel E. Palmer, Dustin A. Dikeman, Toshinori Fujinuma, Brandon Kim, JoshuaI. Jones, Miwako Denda, José Miguel Martínez-Zapater and Marilyn Cruz-Alvarez

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 4, pages 404-413
Published in print April 2001 | ISSN: 0032-0781
Published online April 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce050
A Brassica oleracea Gene Expressed in a Variety-Specific
Manner May Encode a Novel Plant Transmembrane
Receptor

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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The species Brassica oleracea includes several agricultural varieties characterized by the proliferation of different types of meristems. Using a combination of subtractive hybridization and PCR (polymerase chain reaction) techniques we have identified several genes which are expressed in the reproductive meristems of the cauliflower curd (B. oleracea var. botrytis) but not in the vegetative meristems of Brussels sprouts (B. oleracea var. gemmifera) axillary buds. One of the cloned genes, termed CCE1 (CAULIFLOWER CURD EXPRESSION 1) shows specific expression in the botrytis variety. Preferential expression takes place in this variety in the meristems of the curd and in the stem throughout the vegetative and reproductive stages of plant growth. CCE1 transcripts are not detected in any of the organs of other B. oleracea varieties analyzed. Based on the nucleotide sequence of a cDNA encompassing the complete coding region, we predict that this gene encodes a transmembrane protein, with three transmembrane domains. The deduced amino acid sequence includes motifs conserved in G-protein-coupled receptors (GPCRs) from yeast and animal species. Our results suggest that the cloned gene encodes a protein belonging to a new, so far unidentified, family of transmembrane receptors in plants. The expression pattern of the gene suggests that the receptor may be involved in the control of meristem development/arrest that takes place in cauliflower.

Keywords: Key words: Brassica oleracea — Cauliflower — G-protein-coupled receptors — Meristems — Transmembrane proteins.; Abbreviations: FMI, floral meristem identity; GPCR, G-protein-coupled-receptor; LRR, leucine-rich repeat; RACE, rapid amplification of cDNA ends; SSC, saline sodium citrate; SAM, shoot apical meristem; TM, transmembrane.; The nucleotide sequence reported in this paper has been submitted to GenBank under accession number AF227978 (CCE1-cDNA).

Journal Article.  7266 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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