Journal Article

The Establishment of Conditions to Efficiently Screen Photosynthesis-Deficient Mutants of<i> Synechocystis</i> sp. PCC 6803 by Nitrofurantoin Treatment

Akihiro Yamasato and Kimiyuki Satoh

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 4, pages 414-418
Published in print April 2001 | ISSN: 0032-0781
Published online April 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce051
The Establishment of Conditions to Efficiently Screen
Photosynthesis-Deficient Mutants of Synechocystis sp. PCC
6803 by Nitrofurantoin Treatment

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A method based on the susceptibility of photosynthetic organisms to nitrofurantoin under illumination was established to screen mutants of Synechocystis sp. PCC 6803 deficient in the function of photosystem II, which were created by random PCR mutagenesis targeted to the psbAII gene coding for the D1 protein of the photosystem II reaction center. In this method, cyanobacterial colonies on a nitrocellulose membrane on a BG11 agar plate were treated with nitrofurantoin at 1.0 mM under white light at 40 µE m–2 s–1 for 2 h, and then kept under normal conditions without nitrofurantoin so that surviving cells could grow. This method was also shown to be useful for screening mutants deficient in the function of photosystem I.

Keywords: Key words: Nitrofurantoin — Photosystem II — psbA gene — Random mutagenesis — Screening — Synechocystis sp. PCC 6803.; Abbreviations: EPR, Electron Paramagnetic Resonance; FT-IR, Fourier Transform Infrared.

Journal Article.  3330 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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