Journal Article

Chlorophyll Degradation in a <i>Chlamydomonas reinhardtii</i>Mutant: An Accumulation of Pyropheophorbide <i>a</i> by Anaerobiosis

Michio Doi, Tetsuhiko Inage and Yuzo Shioi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 5, pages 469-474
Published in print May 2001 | ISSN: 0032-0781
Published online May 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce057
Chlorophyll Degradation in a Chlamydomonas reinhardtiiMutant: An Accumulation of Pyropheophorbide a by
Anaerobiosis

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Chlorophyll degradation was investigated in cells of a chlorophyll b-less mutant of Chlamydomonas reinhardtii under aerobic and anaerobic conditions. During degradation of chlorophyll under anaerobic conditions, chlorophyll catabolite P535, an open-tetrapyrrole, was not excreted, but pyropheophorbide a was accumulated as the end product with a transient accumulation of chlorophyllide a and pheophorbide a in cells, in contrast to the breakdown under aerobic conditions. It is likely that in the absence of oxygen, degradation of chlorophyll a proceeds to pyropheophorbide a by three consecutive reactions, dephytylation, metal-releasing and demethoxycarbonylation, and then stops due to a limitation of the oxygen that the monooxygenase reaction requires for bilin formation. A novel enzyme catalyzing demethoxycarbonylation of pheophorbide a was partially purified. The enzyme activity increased dependent on the age of cells, and its increase was completely suppressed by cycloheximide. Production of P535 was also dependent on cytoplasmic protein synthesis.

Keywords: Key words: Chlamydomonas reinhardtii mutant — Chlorophyll degradation —Green alga— Pyropheophorbide a accumulation.

Journal Article.  4514 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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