Journal Article

Modulation in the Activity of Purified Tonoplast H<sup>+</sup>-ATPase by Tonoplast Glycolipids Prepared from Cultured Rice (<i>Oryza sativa</i> L. var. Boro) Cells

Mineo Yamaguchi and Kunihiro Kasamo

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 5, pages 516-523
Published in print May 2001 | ISSN: 0032-0781
Published online May 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce064
Modulation in the Activity of Purified Tonoplast
H+-ATPase by Tonoplast Glycolipids Prepared from Cultured
Rice (Oryza sativa L. var. Boro)
Cells

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Glycolipids, phospholipids, and neutral lipids were extracted from the tonoplast fraction of cultured rice cells (Oryza sativa L. var. Boro). Acyl steryl glucoside (ASG) and glucocerebroside (GlcCer) were also prepared from this fraction. We determined the effects of these tonoplast lipids on the activity of H+-ATPase which was delipidated and purified from the tonoplast fraction. Exogenously added tonoplast phospholipids stimulated the activity of purified tonoplast H+-ATPase, but tonoplast glycolipids did not. When tonoplast glycolipids or tonoplast ASG was added in the presence of tonoplast phospholipids, they decreased the phospholipid-induced activation of the tonoplast H+-ATPase; tonoplast GlcCer only caused a small decrease. Steryl glucoside (SG) did not cause any decrease in this activation. Phospholipids, ASG, and GlcCer made up 35 mol%, 20 mol% and 7 mol% of the total lipids of the tonoplast fraction of cultured rice cells, respectively, and these glycolipid levels were enough to depress the phospholipid-induced activation of the tonoplast H+-ATPase. These results revealed that H+-ATPase activity in the tonoplast may be modulated toward activation and depression by tonoplast phospholipids and glycolipids, respectively. The acylation of SG would be responsible for the depression in the phospholipid-induced H+-ATPase activity.

Keywords: Key words: Acyl steryl glucoside — Glucocerebroside — Oryza sativa L.— Tonoplast glycolipids — Tonoplast H+-ATPase (EC 3.6.3.14) — Tonoplast phospholipids.; Abbreviations: ASG, acyl steryl glucoside; DGDG, digalactosyl diacylglycerol; DTT, ditiothreitol; EGTA, ethyleneglycol-bis(β-aminoethylene)-N,N′-tetraacetic acid; GalCer, galactocerebroside; GlcCer, glucocerebroside; MGDG, monogalactosyl diacylglycerol; MOPS, 3-(N-morpholino)-propanesulfonic acid; PA, phosphatidic acid; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PS, phosphatidylserine; SG, steryl glucoside.

Journal Article.  6127 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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