Journal Article

Association of Glutathione with Flowering in <i>Arabidopsis</i> <i>thaliana</i>

Ken’ichi Ogawa, Yasushi Tasaka, Masanobu Mino, Yoshikazu Tanaka and Masaki Iwabuchi

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 5, pages 524-530
Published in print May 2001 | ISSN: 0032-0781
Published online May 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce065
Association of Glutathione with Flowering in Arabidopsis
thaliana

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  • Biochemistry
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In order to study the relationship between GSH and flowering, wild-type and late-flowering mutant, fca-1, of Arabidopsis thaliana were treated with l-buthionine sulfoximine (BSO), a specific inhibitor of GSH biosynthesis, under long-day conditions. BSO treatment of the fca-1 mutant starting at 17 d after imbibition promoted flowering. However, when the treatment was started at 12 d after imbibition, BSO treatment at 10–4 M resulted in an inhibition of flowering. This inhibitory effect of BSO on flowering was abolished by GSH treatment at 10–4 M, although GSH treatment at an increased concentration of 10–3 M clearly delayed flowering. In contrast, BSO treatment of wild-type plants starting at 12 d after imbibition promoted flowering, whose effect was abolished by GSH application. In the fca-1 mutant, whose endogenous GSH levels were high, chilling treatment lowered the GSH levels and promoted flowering, as was the case in the BSO treatment. An A. thaliana mutant, cad2-1, which has a defect in GSH biosynthesis also exhibited late flowering. The late-flowering phenotype of this mutant tended to be strengthened by BSO and abolished by GSH treatment. These results suggest that flowering is associated with the rate of GSH biosynthesis and/or the levels of GSH in A. thaliana.

Keywords: Key words: Arabidopsis thaliana — Buthionine sulfoximine — Differentiation — Flowering — Glutathione — Redox state.; Abbreviation: BSO, l-buthionine sulfoximine.

Journal Article.  4377 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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