Journal Article

Characterization of cDNA of the Liverwort Phytochrome Gene, and Phytochrome Involvement in the Light-Dependent and Light-Independent Protochlorophyllide Oxidoreductase Gene Expression in <i>Marchantia paleacea</i> var. <i>diptera</i>

Takanori Suzuki, Susumu Takio, Isamu Yamamoto and Toshio Satoh

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 6, pages 576-582
Published in print June 2001 | ISSN: 0032-0781
Published online June 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce070
Characterization of cDNA of the Liverwort Phytochrome Gene, and Phytochrome Involvement in the Light-Dependent and Light-Independent Protochlorophyllide Oxidoreductase Gene Expression in Marchantia paleacea var. diptera

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The cDNA of the phytochrome gene in the liverwort Marchantia paleacea var. diptera (MpdPHY1) was isolated. MpdPHY1 encoded a conventional phytochrome apoprotein. The MpdPHY1 transcript was accumulated in the dark and suppressed in the light. The degradation of the MpdPHY1 transcript by red light irradiation had red/far-red reversibility, suggesting that the liverwort phytochrome gene expression was regulated by a phytochrome. Northern blot analysis of the transcripts in cells irradiated by red/far-red light revealed that the liverwort phytochrome was involved in the expressions of chlB, chlL, chlN, or por, which encode subunits of light-independent and light-dependent protochlorophyllide oxidoreductase, respectively.

Keywords: Key words: Chlorophyll synthesis — Liverwort (Marchantia paleacea) — Photoregulation — Phytochrome —Protochlorophyllide oxidoreductase (EC 1.6.99.1).; Abbreviations: DPOR, light-independent POR; LPOR, light-dependent POR; ORF, open reading frame; phy, holoprotein of phytochrome; PHY, apoprotein of phytochrome; PHY, phytochrome gene; POR, protochlorophyllide oxidoreductase; RACE, rapid amplification of cDNA ends; RT-PCR, reverse transcription-PCR; UTR, untranslated region.

Journal Article.  5138 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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