Journal Article

Spatial and Temporal Distribution of Sucrose Synthase in the Radish Hypocotyl in Relation to Thickening Growth

Hervé Rouhier and Hideaki Usuda

in Plant and Cell Physiology

Published on behalf of Japanese Society of Plant Physiologists

Volume 42, issue 6, pages 583-593
Published in print June 2001 | ISSN: 0032-0781
Published online June 2001 | e-ISSN: 1471-9053 | DOI: http://dx.doi.org/10.1093/pcp/pce071
Spatial and Temporal Distribution of Sucrose Synthase in the Radish Hypocotyl in Relation to Thickening Growth

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  • Biochemistry
  • Molecular and Cell Biology
  • Plant Sciences and Forestry

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Sucrose synthase (SuSy) is a key enzyme in the development of storage root of radish. Clarification of its spatial and temporal expression during the thickening growth of radish hypocotyl, which later develops into storage root, was carried out immunologically using light microscopy. Sequential harvests at 3, 7, 11 and 13 d after sowing (DAS) were performed on two radish cultivars having different sink capacity. A very low level of SuSy was observed 3 DAS for both cultivars. White Cherrish (WC; strong storage root) showed the maximum level of SuSy between 7 and 11 DAS with increased cell development (thickening), while in Kosena (K; low storage root) the level remained high after 13 d of growth. A high level of SuSy was found in companion cells, which was consistent with previous observations, but SuSy was also found in the xylem parenchyma and in some cortical cells. The level of SuSy differed according to the localization and depended highly on cell development. Both cell division and cell enlargement were stimulated in WC compared with K. The role of SuSy during thickening growth of radish hypocotyl is discussed in terms of utilizing photosynthates.

Keywords: Key words: Immuno-cytochemistry — Kosena — Raphanus sativus L. — Storage root — Sucrose synthase — White Cherrish.; Abbreviations: DAS, day after sowing; K, Kosena; PBS, phosphate buffered saline; PBST, PBS with 0.1% (v/v) Tween 20; SuSy, sucrose synthase; WC, White Cherrish.

Journal Article.  5879 words.  Illustrated.

Subjects: Biochemistry ; Molecular and Cell Biology ; Plant Sciences and Forestry

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